Ex vivo perfusion pharmacology study· 1989
Saturable mechanism for delta sleep-inducing peptide (DSIP) at the blood-brain barrier of the vascularly perfused guinea pig brain
Zlokovic BV; Susic VT; Davson H; Begley DJ; Jankov RM; Mitrovic DM
Peptides
- PubMed
- 2547200
- Evidence level
- laboratory
- Verification
- verified
Summary
Zlokovic et al. 1989 demonstrate a high-affinity saturable transport mechanism for DSIP across the blood–brain barrier of the perfused guinea-pig brain: cold DSIP 7 μM reduces [125I]-DSIP Kin to mannitol-like values; L-tryptophan (DSIP's N-terminal residue) inhibits Kin at physiological concentrations; Arg8-vasopressin inhibits only at 0.2 mM.
Limitations
Ex-vivo perfused-brain preparation only; single species (guinea pig); no in-vivo brain-penetration confirmation; older methodology; small dataset described in abstract.